Sub Programmes

WP 2, Training-through-research, WP Leader; University of Cambridge

Sub Programme 1 (SP1)
Title: Effects of maternal diabetes and/or obesity on early embryo metabolism
SP leader: Martin-Luther-Universitaet 
Partners: Biotalentum, Avantea, Szent Istvan University, MWM Biomodels
ESR/ER's trained: ESR3, ESR7, ESR10, ESR11, ESR12, ER4
Milestones
SP1A: The rabbit embryo model. Study the mechanisms of metabolic programming by the insulin/IGF, PPAR/AMPK, mTOR, and AGE/RAGE axes and DNA and protein methylation in embryos derived from experimentally induced diabetic rabbits.
SP1B: Cellular models. Study the mechanisms of metabolic programming in mouse, rabbit and human ES, EpiS and iPS cells.
SP1C: The porcine diabetes model. Study the mechanism of metabolic programming by the incretin glucose-dependent insulinotropic polypeptide (GIP) in transgenic pigs expressing a dominant-negative GIP receptor (GIPRdn) in pancreatic islets.

Sub Programme 2 (SP2)
Title: Human ART and adult health, including modelling in animal in vivo and human in vitro systems.
SP leader: University of Southampton
Partners: Utrecht University, University of Cambridge, University of Manchester, Szent Istvan University
Sub Partners:  Biotalentum, Avantea
ESR/ER's trained: ESR1, ESR2, ESR4, ESR8, ESR9
Milestones
SP2A: Mouse, porcine and equine embryo and cellular modelUtilise animal models of preimplantation embryo environmental programming , determination of gene pathways, metabolic signalling and quality parameters affected in the short-term  and longer-term.
SP2B: Human ART modelTo examine the impact of ART (age of embryo donor, embryo culture length, cryopreservation) on early human embryo development, pregnancy outcome and health of offspring from perinatal to early adulthood. To examine the impact of periconception diet and ART technologies on ART outcomes using existing databases, and the impact of ART on perinatal and child health by linking to national disease databases.
 
Sub Programme 3 (SP3)
TitlePericonception epigenetic and imprinting programming mechanism in knock-out mouse and stem cell models
SP leader: University of Cambridge
PartnersAvantea
Sub Partners:  University of Manchester, Biotalentum
ESR/ER's trained: ESR5, ER3
Milestones
The identification and role of epigenetic stability factors in normal and compromised ES cells. Epigenetic stability in ES, EpiS and iPS cells.
 
Sub Programme 4 (SP4)
Title: Data mining, bioinformatics analyses and modelling of pathways and intervention methods
SP leader: University of Cambridge
Partners: Biotalentum 
Sub Partners:  University of Southampton, University of Manchester
ESR/ER's trained: ESR6, ER1
Milestones
SP4A: Mouse, Data mining and bioinformaticsData mining on EpiHealthNet, HFEA, Brison/Macklon databases, bioinformatics analyses of microarray, sequencing and metabolomics data from SP1, 2, 3.
SP4B: Modelling and development of epigenetic and metabolic intervention methods for healthy periconception developmentBased on SP4A and SP3 results, design of strategies to modulate key pathways by changing in vitro culture conditions or maternal nutrition. Evaluation of KRAB protein use for potential intervention.